Calu-3 Human Lung Adenocarcinoma Cell Line

Lung cancer is the most common culprit of cancer-related deaths in the world.1 Even with advances in technology as well as treatment, it remains incredibly prevalent. Adenocarcinoma, which begins in the mucus producing cells of the body, is the most common sub-categorization of primary lung cancer. It accounts for about 40% of all cases of lung cancer.2 Adenocarcinoma is highly aggressive and is usually diagnosed at more advanced stages, resulting in low survival rates.
Adenocarcinoma presents a variety of challenges due to its resistance to common treatments such as radiotherapy and chemotherapy. Combining multiple forms of therapy helps to overcome these roadblocks. The Calu-3 cell line can assist in finding potential drug therapies and has shown to be useful in mimicking in vivo conditions.3 This similarity has allowed for many pharmaceutical applications such as drug transport, studying aerosol deposition, controlled release studies, and identification of potential drug-drug interactions.4 The characteristics of Calu3 cell line allow for a basic in vitro method of developing new therapeutics in an epithelial respiratory model.

Source
The Calu-3 cell line was established by isolating cells located in the pleural effusion of a 25-year-old Caucasian man with lung adenocarcinoma.5

References
1. Semin Ultrasound CT MR 2019, 40(3): 255-264.
2. Cell Death Dis 2018, 9(2): 117.
3. J Control Release 2003, 87(1-3): 131-138.
4. Expert Opin Drug Deliv 2013, 10(9): 1287-1302.
5. J Natl Cancer Inst 1977, 59(1): 221-226.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The characteristics of Calu3 cell line allow for a basic in vitro method of developing new therapeutics in an epithelial respiratory model.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.