Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line

Huh-7 is a well differentiated hepatocyte-derived carcinoma cell line, isolated from the liver tumor of a 57-year-old Japanese male (Nakabayashi, 1982). Derived from Huh-7, the Huh-7.5 cell line is highly permissive for subgenomic and genomic Hepatitis C virus (HCV) RNA replication (Blight et al., 2002) and is now employed for high yield production of HCV and studies of antivirals on industrial scale. Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line was generated from Huh-7.5 cells using retroviral transduction to express the Tet-On 3G transactivator protein and enable subsequent doxycycline-regulated transgene expression to aid in the study of subcellular interactions between HCV and its host (Luna, 2015; Takacs, 2017).

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Huh-7.5 Tet-On human hepatocellular carcinoma cell line is useful for Hepatitis C virus (HCV) studies and production.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.