ECM815
Osteogenesis Quantitation Kit
The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.
別名:
Bone Formation Kit, Osteogenesis Detection Kit
製品名
Osteogenesis Quantitation Kit, The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.
manufacturer/tradename
Chemicon®
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
Quality Level
Application
Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.
Research Category
Cell Structure
Cell Structure
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CHEMICON′s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.
The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.
For Research Use Only. Not for use in diagnostic procedures.
The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.
For Research Use Only. Not for use in diagnostic procedures.
Other Notes
Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C.
10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. 80% acetic acid is not included with the kit. Store at 2-8°C.
10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. 80% acetic acid is not included with the kit. Store at 2-8°C.
Preparation Note
All components should be stored at 2 - 8ºC until expiration date printed on label.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
保管分類
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Xiang Fang et al.
PloS one, 9(2), e88874-e88874 (2014-03-04)
A number of publications have reported that adipose derived stem cells (ADSCs) have the capacity to be induced to differentiate into osteoblasts both in vitro and in vivo. However, it has been difficult to use separate ADSCs for cortical bone
Mona Alsulaiman et al.
Journal of cell communication and signaling, 10(1), 17-31 (2015-12-03)
Lysyl oxidase pro-enzyme is secreted by tumor cells and normal cells as a 50 kDa pro-enzyme into the extracellular environment where it is cleaved into the ~30 kDa mature enzyme (LOX) and 18 kDa pro-peptide (LOX-PP). Extracellular LOX enzyme activity is required for
Florian Andreas Probst et al.
Scientific reports, 10(1), 2062-2062 (2020-02-08)
Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used for bone regeneration facilitating the formation of bone extracellular matrix
Junya Yoshitani et al.
Scientific reports, 10(1), 11182-11182 (2020-07-09)
Implant-related infection is difficult to treat without extended antibiotic courses. However, the long-term use of antibiotics has led to the development of multidrug- and methicillin-resistant Staphylococcus aureus. Thus, alternatives to conventional antibiotic therapy are needed. Recently, mesenchymal stem cells have
Jifeng Xu et al.
Aging, 13(15), 19282-19292 (2021-08-11)
Osteoporosis is a common aging-related disease diagnosed primarily using bone mineral density (BMD). Extracellular vesicles (EVs) remain unexplored in the context of osteoporosis. Towards this, EVs were isolated from plasma of a discovery cohort with 8 non-osteoporotic and 8 osteoporotic
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| ECM815 | 04053252470837 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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