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Merck

CC080

Human Vitronectin

from human plasma, liquid, 1 mg/mL, suitable for cell culture

別名:

S-protein

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この商品について

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Assay:
≥90% (SDS-PAGE)

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製品名

Vitronectin, Human Purified Protein,

biological source

human

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

1 μg/mL (cell attachment assays)

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

NCBI accession no.

NM_000638.3

UniProt accession no.

P04004

shipped in

dry ice

storage temp.

−70°C

Quality Level

100

Gene Information

human ... VTN(7448)

関連するカテゴリー

Analysis Note

Two bands in SDS-PAGE at 65 kDa and 75 kDa.

Application

Suitable for use in ELISA, immunization, cell attachment assays and electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PROPERTIES:

Binds to heparin. Active in cell attachment of MG63 cells.
Product Source: Human plasma; tested negative for antibodies to HBsAg and HIV.
Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.

Other Notes

Replaces: 08-126

Physical form

Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.

Preparation Note

Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

試験成績書（COA）

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以前この製品を購入いただいたことがある場合

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Jian Li et al.
Biointerphases, 5(3), FA132-FA142 (2010-12-22)
The standard method for culturing human embryonic stem cells (hESC) uses supporting feeder layers of cells or an undefined substrate, Matrigel(™), which is a basement membrane extracted from murine sarcoma. For stem cell therapeutic applications, a superior alternative would be
Lynn Yap et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 106(5), 1887-1896 (2017-09-25)
Functionalizing medical devices with polypeptides to enhance their performance has become important for improved clinical success. The extracellular matrix (ECM) adhesion protein vitronectin (VN) is an effective coating, although the chemistry used to attach VN often reduces its bioactivity. In
Carolina Epifano et al.
EMBO reports, 15(5), 592-600 (2014-03-19)
The adherens junction protein p120-catenin is implicated in the regulation of cadherin stability, cell migration and inflammatory responses in mammalian epithelial tissues. How these events are coordinated to promote wound repair is not understood. We show that p120 catenin regulates
Morten Aagaard Nielsen et al.
Frontiers in immunology, 13, 915890-915890 (2022-07-12)
4-1BB is a T cell costimulatory receptor and a member of the tumor necrosis factor receptor superfamily. Here, we show that Galectin-3 (Gal-3) decreases the cellular response to its ligand (4-1BBL). Gal-3 binds to both soluble 4-1BB (s4-1BB) and membrane-bound
Andrew J Putnam et al.
Cell communication and signaling : CCS, 7, 10-10 (2009-04-30)
Integrins, cell-surface receptors that mediate adhesive interactions between cells and the extracellular matrix (ECM), play an important role in cancer progression. Expression of the vitronectin receptor alphavbeta3 integrin correlates with increased invasive and metastatic capacity of malignant melanomas, yet it
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プロトコル

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

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グローバルトレードアイテム番号

カタログ番号GTIN
CC08004053252590238

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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