AB749P
Anti-Collagen Type I Antibody
Chemicon®, from rabbit
製品名
Anti-Collagen Type I Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... COL1A1(1277)
Application
Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Reacts with bovine collagen type I collagen.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
purified bovine type I collagen extracted from purified bovine skin.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB749
Physical form
Format: Purified
Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.
Preparation Note
Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Lucio Díaz-Flores et al.
Scientific reports, 10(1), 4987-4987 (2020-03-21)
In lobular capillary hemangioma (LCH), misnamed pyogenic granuloma, only sprouting angiogenesis (SA) has been considered. We assess the occurrence of intussusceptive angiogenesis (IA) in LCH and whether IA determines the specific and other focal patterns in the lesion. For this
Telocytes/CD34+ Stromal Cells in the Normal, Hyperplastic, and Adenomatous Human Parathyroid Glands.
Lucio Díaz-Flores et al.
International journal of molecular sciences, 24(15) (2023-08-12)
Telocytes/CD34+ stromal cells (TCs/CD34+ SCs) have been studied in numerous organs and tissues, but their presence and characteristics in the parathyroid glands have not been explored. Using immunological and ultrastructural procedures, we assess the location, arrangement, and behavior of TCs/CD34+
Sarah E Gullbrand et al.
Acta biomaterialia, 70, 154-164 (2018-02-11)
Replacement of the intervertebral disc with a viable, tissue-engineered construct that mimics native tissue structure and function is an attractive alternative to fusion or mechanical arthroplasty for the treatment of disc pathology. While a number of engineered discs have been
Cemile Bektas et al.
Bioengineering (Basel, Switzerland), 11(8) (2024-08-31)
Cultivated meat, an advancement in cellular agriculture, holds promise in addressing environmental, ethical, and health challenges associated with traditional meat production. Utilizing tissue engineering principles, cultivated meat production employs biomaterials and technologies to create cell-based structures by introducing cells into
Lucio Díaz-Flores et al.
Histology and histopathology, 36(2), 217-228 (2020-12-19)
Intussusceptive angiogenesis (IA) is the process by which pre-existing blood vessels split, expand and remodel through intravascular pillar formation. In previous works, we studied the morphologic characteristics of intravascular papillary endothelial hyperplasia (IPEH) and suggested the participation of IA in
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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