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Merck

AB10181

Anti-Pumilio-2 (PUM2) Antibody

from rabbit, purified by affinity chromatography

別名:

pumilio homolog 2 (Drosophila), pumilio homolog 2, pumilio (Drosphila) homolog 2

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunohistochemistry
western blot
Species reactivity:
rhesus macaque, mouse, human
Citations:
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
Q8TB72

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製品名

Anti-Pumilio-2 (PUM2) Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rhesus macaque, mouse, human

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_056132

UniProt accession no.

Q8TB72

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PUM2(23369)

Application

Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.
Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
RNA Metabolism & Binding Proteins

Analysis Note

Control
Mouse embryonic stem cell lysate
Evaluated by Western Blot on mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.

Biochem/physiol Actions

This antibody recognizes Pumilio-2 near the N-terminus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.
~120 kDa observed.
Non specific band may be observed at 48 kDa

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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保管分類

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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グローバルトレードアイテム番号

カタログ番号GTIN
AB1018104053252478475

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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