1.19613
Superspher® 100 RP-18 (4 µm) HPLC Columns
HPLC sorbent
別名:
TM=["Superspher"] C18 sorbent for HPLC
製品名
Superspher® 100 RP-18,
material
stainless steel column
agency
suitable for USP L1
product line
Superspher®
feature
endcapped: no
parameter
60 °C max. temp.
technique(s)
HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)
matrix
fully porous particle
matrix active group
C18 bonding phase
particle size
4 μm
pore size
100 Å pore size
operating pH range
2-7.5
bulk density
200‑400 kg/m3
separation technique
reversed phase
storage temp.
no temp limit
Analysis Note
Application test: passes test
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0
Application
- Determination of retinol and retinyl esters in human plasma: This study utilized high-performance liquid chromatography with automated column switching and ultraviolet detection, highlighting the utility of the Superspher 100 RP-18 HPLC column in sensitive biochemical analyses (Hartmann et al., 2001).
- Enantioselective assay for ketoprofen glucuronidation: This study underscores the column′s capability in pharmacokinetic research, using HPLC to analyze chiral anti-inflammatory drugs and their metabolites in liver microsomes (Chakir et al., 1994).
- Rapid liquid chromatographic assay of glutathione in cultured cells: Employing the Superspher 100 RP-18 column, this research focused on rapid and effective glutathione quantification, demonstrating the column′s versatility in biomedicine (Leroy et al., 1993).
- Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography: This application further exemplifies the adaptability of Superspher® 100 RP-18 in biotechnology, offering precise measurement techniques for enzymatic activity studies (Burzio et al., 1988).
General description
Superspher® RP-18 is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.
Legal Information
Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 1.19613.0010 | 04022536178950 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）