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Merck

1.11755

PALCAM Agar (Base)

NutriSelect® Prime, according to ISO 11290, FDA-BAM, powder, selective for Listeria spp., pkg of 500 g

別名:

Listeria selective agar

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この商品について

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
NutriSelect® Prime
Agency:
FDA-BAM
according to ISO 11290
Ph:
6.9-7.1 (25 °C, 68.8 g/L in H2O, after autoclaving)
Form:
powder
Suitability:
selective for Listeria spp.
Application(s):
food and beverages
microbiology

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製品名

PALCAM Agar (Base), NutriSelect® Prime, according to ISO 11290, FDA-BAM, powder, selective for Listeria spp., pkg of 500 g

agency

FDA-BAM
according to ISO 11290

sterility

non-sterile

form

powder

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Prime

technique(s)

microbiological culture: suitable

pH

6.9-7.1 (25 °C, 68.8 g/L in H2O, after autoclaving)

solubility

68.8 g/L

bulk density

650 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

selective for Listeria spp.

Quality Level

200

関連するカテゴリー

Analysis Note

Base tested with PALCAM-Listeria selective supplement acc. to VAN NETTEN et.al. art. no. 1.12122.
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Application

GranuCult® PALCAM agar (Base) is suitable for the detection and isolation of Listeria monocytogenes from feces, biological samples, foodstuffs, and heavily contaminated material from the environment.

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

GranuCult® PALCAM agar (Base) provides quantitative cultivation of Listeria monocytogenes and inhibits the accompanying Gram-negative and most of the Gram-positive bacteria. Polymyxin, acriflavin, ceftazidime, and lithium chloride result in the selectivity of the medium. L. monocytogenes break down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron (III) ions, which stains the colonies of L. monocytogenes. Mannitol-positive accompanying bacteria such as Staphylococci grow as yellow colonies if they are not inhibited.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

保管分類

11 - Combustible Solids

wgk

WGK 3

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資料

Types of Media in Microbiology

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

食品・環境試料中のListeria monocytogenes（リステリア・モノサイトゲネス）の検出

ISO 11290-1、FDA-BAMおよびUSDA-FSISに則った食品・環境試料中のリステリア・モノサイトゲネスの検出

プロトコル

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Discover the revised EN ISO 11290-1 and EN ISO 11290-2 standards and gain insights into updated detection methods for Listeria monocytogenes and Listeria spp., including enumeration in food, animal feed, and environmental samples. Explore culture media solutions.

グローバルトレードアイテム番号

カタログ番号GTIN
1.11755.050004022536135625

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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