Enterobacteria Enrichment (EE) Broth Mossel

GranuCult^® prime Enterobacteriaceae Enrichment Broth - Mossel is designed for selective enrichment of bile-tolerant, Gram-negative bacteria from non-sterile pharmaceutical products. D (+) Glucose enhances the growth of all Lactose-positive and -negative Enterobacteriaceae. The undesired, accompanying bacterial flora is completely inhibited by brilliant green and ox bile. The strong buffering capacity of the culture medium prevents the formed acid from killing the culture.

GranuCult^® prime Enterobacteriaceae Enrichment Broth - Mossel is suitable for sterility testing of substances, preparations, and products as well as a pre-enrichment broth for the test for specified microorganisms or total viable aerobic count determinations using the MPN method within non-sterile pharmaceutical products.

Appearance (clearness): clear
Appearance (colour): green
pH-value (25 °C): 7.0 - 7.4
Typical composition (g/litre): Peptone from Gelatine 10.0; D(+)-Glucose 5.0; Ox bile dried 20.0; Brilliant green 0.015; di-Sodium hydrogen phosphate dihydrate 8.0; Potassium dihydrogen phosphate 2.0.
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): 10 - 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 100
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): +
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): no growth
Incubation: 24 hrs.; 30-35 °C; S. aureus 48 hrs.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.