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Merck

PC205L

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

lyophilized, Calbiochem®

別名:

Anti-CGRP

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この商品について

NACRES:
NA.43
UNSPSC Code:
12352203
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製品名

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb, lyophilized, Calbiochem®

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Free Floating Sections
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200,
Paraffin Sections (s; heat pre-treatment required; ))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

mouse ... Calca(12310)

Analysis Note

Positive Control
Rat amygdala or spinal cord

Application


Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.

Immunogen

rat α-calcitonin gene related peptide (α-CGRP)

Other Notes

Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.

Preparation Note

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Min-Hee Oh et al.
Journal of immunology (Baltimore, Md. : 1950), 191(11), 5371-5382 (2013-10-22)
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D J Van Daele et al.
Neuroscience, 162(2), 501-524 (2009-05-12)
The present study investigated the central connections of motor neurons innervating the thyroarytenoid laryngeal muscle that is active in swallowing, respiration and vocalization. In both intact and sympathectomized rats, the pseudorabies virus (PRV) was inoculated into the muscle. After initial
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Although there are many candidates as molecular mechanotransducers, so far there has been no evidence for molecular specialization of visceral afferents. Here, we show that colonic afferents express a specific molecular transducer that underlies their specialized mechanosensory function: the transient
Liron S Duraku et al.
Molecular pain, 8, 61-61 (2012-09-01)
The epidermis is innervated by fine nerve endings that are important in mediating nociceptive stimuli. However, their precise role in neuropathic pain is still controversial. Here, we have studied the role of epidermal peptidergic nociceptive fibers that are located adjacent
Francis M Hughes et al.
Diabetes, 68(2), 430-440 (2018-11-15)
The NLRP3 inflammasome senses diabetic metabolites and initiates inflammation implicated in diabetic complications and neurodegeneration. No studies have investigated NLRP3 in diabetic bladder dysfunction (DBD), despite a high clinical prevalence. In vitro, we found that numerous diabetic metabolites activate NLRP3

グローバルトレードアイテム番号

カタログ番号GTIN
PC205L-100ULCN04055977209532

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