574795
TBE Buffer, 10X, Molecular Biology Grade
A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
別名:
Tris-Borate-EDTA Buffer
製品名
TBE Buffer, 10X, Molecular Biology Grade, A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
grade
Molecular Biology
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
foreign activity
DNase, RNase, protease, none detected
shipped in
ambient
storage temp.
15-25°C
関連するカテゴリー
Application
TBE Buffer, 10X, Molecular Biology Grade-Calbiochem has been used as a component of polyacrylamide sequencing gels for DNA electrophoresis using amplified fragment length polymorphism (AFLP).
Disclaimer
Toxicity: Irritant (B)
General description
A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COO- species). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg2+ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments. It also stabilizes nucleic acids against enzymatic degradation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
保管分類
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Marjan Sadat Hosseini et al.
Phytochemistry, 183, 112629-112629 (2021-01-31)
Licorice (Glycyrrhiza glabra L.) is a medicinal plant species valued in many countries in Asia and Europe for its phytochemical characteristics. Licorice biodiversity is becoming threatened nowadays in Iran due to increasing demand and a drastic decline of its natural
Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 574795-1LCN | 04055977189773 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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