Ready-to-Assay GAL₂ Galanin Receptor Frozen Cells

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Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Galanin is a 29-30 amino acid peptide originally purified from intestine, but later found to be abundant in the CNS. It is widely distributed in tissues such as the brain, spinal cord and gut, and can regulate numerous processes including feeding, nociception, nerve regeneration, memory, neuroendocrine release, and gut secretion and contractility. Galanin elicits its physiological effects through the stimulation of at least three G protein-coupled receptors (Branchek et al. 2000). GAL2 receptor couples predominantly to the activation of phospholipase C. It plays an important role in modulating neurite outgrowth and has been demonstrated to be the principal receptor subtype that mediates the protective effects of galanin in the hippocampus (Elliott-Hunt et al. 2007). Millipore′s cloned human GAL2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GAL2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GAL2 Receptor.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.