form
solid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
microbiological culture: suitable
application(s)
microbiology
shipped in
ambient
storage temp.
10-30°C
Quality Level
Application
Overnight Express Instant TB Medium – Novagen has been used:
- to culture recombinant Escherichia coli colonies for protein expression.
- as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production
- as a medium for hamster recombinant PrP (rPrP) protein expression
Disclaimer
Toxicity: Standard Handling (A)
Features and Benefits
- Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
- Rich culture media: high cell densities and protein expression level
- Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
- Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
- Convenient, no need to spend extra time on weighting out different ingredients
General description
Overnight Express™ Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.
Preparation Note
The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.
Legal Information
Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
11 - Combustible Solids
wgk
WGK 3
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of
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Biomaterials, 31(9), 2695-2700 (2009-12-29)
Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins. In contrast the silk of honeybees (Apis mellifera) is composed of a family of four
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Journal of immunoassay & immunochemistry, 33(4), 352-368 (2012-09-12)
RGD peptide (Arg-Gly-Asp tripeptide) binds to integrin αVβ(3) and αVβ(5), which is selectively expressed in tumor neovasculature and on the surface of some tumor cells. Some studies showed that coupling the RGD peptides to anticancer drugs yielded compounds with increased
Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution.
Mancini, et al.
Protein Science, 16, 2294-2300 (2018)
Nariyasu Tahara et al.
Protein and peptide letters, 28(10), 1180-1190 (2021-08-07)
Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 71491-5CN | 04055977270242 |
| 71491-3CN | 04055977270228 |
| 71491-4CN | 04055977270235 |
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