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Merck

MSIPS4W10

Multiscreen®96ウェルプレート、疎水性PVDFメンブレン

pore size 0.45 μm, sterile

別名:

PVDF96 ウェルプレート, 疎水性 96 ウェルプレート

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この商品について

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006

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製品名

Multiscreen®96ウェルプレート、疎水性PVDFメンブレン, pore size 0.45 μm, sterile

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

Quality Level

400

material

Durapore PVDF membrane
flat bottom wells
white acrylic plate

usage

Automated

feature

hydrophobic
lid

packaging

pkg of 10 plates

manufacturer/tradename

MultiScreen®

technique(s)

ELISPOT: suitable
cell based assay: suitable
dot blot: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

compatibility

centrifuge & manifold

shipped in

ambient

関連するカテゴリー

Application

MultiScreen® HTS IPフィルタープレート、0.45 μm、白色、無菌は、以下を用いた酵素結合イムノスポット（ELISpot）アッセイに使用されています：
  • dsDNA産生形質細胞
  • メモリーB細胞と骨髄に局在する長寿命形質細胞
  • 流入領域リンパ節と脾臓細胞

General description

捕捉抗体の結合がわずかに改善され、スポット検出、特に蛍光用途で優れた性能を示すことから、混合セルロースエステルよりもポリフッ化ビニリデン（PVDF）メンブレンプレートが推奨されます。

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains

資料

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

プロトコル

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

関連コンテンツ

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

MultiScreen®HTS and MultiScreen®HTS+ Hi Flow Assay Systems
Elispot assays: state-of-the-art tools for functional analysis of cellular immunology White Paper

"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.

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グローバルトレードアイテム番号

カタログ番号GTIN
MSIPS4W1004053252464744

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