MAHAS4510
Multiscreen® 96 well Plate, MCE membrane
pore size 0.45 μm, sterile
別名:
Detection Kit
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この商品について
flat bottom wells
mixed cellulose esters (MCE) membrane
製品名
Multiscreen® 96 well Plate, MCE membrane, pore size 0.45 μm, sterile
description
Sterile, clear 96-well filter plate with 0.45 um pore size Mixed Cellulose Esters membrane for cell culture & ELISPOT Assays. Comes in a pack of 10.
sterility
sterile
Quality Level
material
clear polystyrene plate
flat bottom wells
mixed cellulose esters (MCE) membrane
usage
Manual
feature
hydrophilic
lid
packaging
pkg of 10 plates
manufacturer/tradename
MultiScreen®
technique(s)
DNA purification: suitable
ELISPOT: suitable
activity assay: suitable (with precipitations)
cell based assay: suitable
dot blot: suitable
ligand binding assay: suitable
receptor binding assay: suitable
filtration area
0.28 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
MF-Millipore™
pore size
0.45 μm pore size
application(s)
sample preparation
binding type
high binding surface
compatibility
manifold only
shipped in
ambient
Application
Cell Culture
Assays
Protein
Radionucleotides
General description
HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.
- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.
Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.
Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.
Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
Other Notes
Physical form
Legal Information
保管分類
11 - Combustible Solids
wgk
WGK 3
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
プロトコル
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
関連コンテンツ
"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."
MultiScreen® Filter Plates
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| MAHAS4510 | 04053252577239 |
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