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SCR544 Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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SCR544
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      概要

      Replacement Information
      Description
      Catalogue NumberSCR544
      Trade Name
      • STEMCCA
      DescriptionHuman STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
      OverviewThe Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.
      References
      Product Information
      Components
      • 1. Human STEMCCA Constitutive (OKSM) Lentivirus: (Part number SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Part number CS204502). Each vial contains 15 µL of high titer lentivirus.
      • 2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationThe Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
      Biological Information
      Stem Cell Type
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      SCR544 04053252013805

      Documentation

      Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      カタログ

      タイトル
      Human Stem Cell Systems
      Simplifying the iPSC Workflow
      Stem Cell Research

      技術情報

      タイトル
      Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

      データシート

      タイトル
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
      STEMCCA Lentivirus Reprogramming Kits

      ユーザーガイド

      タイトル
      Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

      関連製品＆アプリケーション

      Accessories

      カタログ番号 説明  
      SCM088 Human iPS Reprogramming Boost Supplement 価格＆在庫状況を表示

      カテゴリー

      Life Science Research > Cell Culture and Systems > Stem Cell Re-Programming (iPS)