AB2272
Anti-ZO-1 Antibody
from rabbit, purified by affinity chromatography
別名:
tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1
製品名
Anti-ZO-1 Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TJP1(7082)
Analysis Note
Control
HepG2 cell lysate
HepG2 cell lysate
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ZO-1 on 10 µg of HepG2 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ZO-1 on 10 µg of HepG2 cell lysate.
Application
Detect ZO-1 using this Anti-ZO-1 Antibody validated for use in WB, IH(P).
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected ZO-1 in normal human testis tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Biochem/physiol Actions
Other homologies: Rat ( 89% sequence homology).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
~260 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human ZO-1.
Other Notes
Concentration: Please refer to lot specific datasheet.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| AB2272 | 04053252645556 |
| AB2272-25UG | 04054839342615 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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