Anti-RBM4 Antibody

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected RBM4 in 10 µg of HepG2 cell lysate.

Anti-RBM4 Antibody is an antibody against RBM4 Antibody for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected RBM4 in HeLa and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RBM4 is a widely expressed RNA-binding factor involved in many cellular process, including alternative splicing of pre-mRNA and translation regulation by modulating alternative 5′-splice site and exon selection on target hnRNA. RBM4 plays roles in development, particularly muscle cell development, clock metabolism and cellular stress. In muscle, RBM4 acts as a muscle cell differentiation-promoting factor and activates exon skipping of the PTB pre-mRNA during muscle cell differentiation, and it antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. RBM4 also mediates various mRNA functions important for myocytes, and RBM4 associates with important miRNAs that are important for muscle cell differentiation. RBM4 is also involved in regulation of the biological clock because it is required for the translational activation of PER1 mRNA in response to circadian clock. In cellular stress, RBM4 recruits EIF4A1 to stimulate IRES-dependent translation initiation in response to cellular stress, and RBM4 associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions.

~42 kDa observed

His-tagged recombinant protein corresponding to human RBM4.

Concentration: Please refer to lot specific datasheet.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.