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NB13 PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1)

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NB13
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with unphosphorylated α subunit of CaM kinase II or with the β subunit in either phosphorylation state.
      Catalogue NumberNB13
      Brand Family Calbiochem®
      Application Data
      Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
      References
      ReferencesMayford, M., et al. 1996. Science 274, 1678.
      Lisman, J. 1994. Trends Neurosci. 17, 406.
      Ochiishi, T., et al. 1994. Brain Res. 659, 179.
      Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Silva, A.J., et al. 1992. Science 257, 201.
      Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesELISA (see comments)
      Immunoblotting (1:2000)
      Immunofluorescence (1:100)
      Application CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
      ImmunogenMouse and Rat
      Clone22B1
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NB13 0

      Documentation

      PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1) Certificates of Analysis

      TitleLot Number
      NB13

      References

      Reference overview
      Mayford, M., et al. 1996. Science 274, 1678.
      Lisman, J. 1994. Trends Neurosci. 17, 406.
      Ochiishi, T., et al. 1994. Brain Res. 659, 179.
      Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Silva, A.J., et al. 1992. Science 257, 201.
      Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:2000)
      Immunofluorescence (1:100)
      Application Data
      Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells. Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated on Thr286.
      BackgroundThe Ca2+/calmodulin-dependent kinase II (CaMKII) is a serine-threonine protein kinase which has been shown to phosphorylate a number of neural substrates including synapsin I, nitric oxide synthase, tryptophan and tyrosine hydroxylase, and the inositol 1,4,5-triphosphate receptors (IP3Rs). CaMKII therefore regulates a broad array of functions including the metabolism of carbohydrates, lipids, and amino acids, neurotransmitter synthesis and release, ion channel and cytoskeletal function, and gene expression. CaMKII is a heterooligomer consisting of two distinct but related isoforms of α and β which are abundantly expressed in neurons of the neocortex, hippocampus, amygdala, and basal ganglia. Following a brief exposure to Ca2+, CaMKII can be converted to a Ca2+-independent form via autophosphorylation of threonine-286, suggesting that CaMKII may play an important physiological role in memory formation. Indeed, both mutant mice deficient in CaMKII, and animals expressing an activated calcium-independent form of CaMKII shows deficiencies in hippocampal long-term potentiation (LTP).
      HostMouse
      Immunogen speciesMouse and Rat
      Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
      Clone22B1
      IsotypeIgG₁
      Speciesrat
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMayford, M., et al. 1996. Science 274, 1678.
      Lisman, J. 1994. Trends Neurosci. 17, 406.
      Ochiishi, T., et al. 1994. Brain Res. 659, 179.
      Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Silva, A.J., et al. 1992. Science 257, 201.
      Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.