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MABF267 Anti-Cochlin Antibody/COCH, clone 9A10D2

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MABF267
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF267
      DescriptionAnti-Cochlin Antibody/COCH, clone 9A10D2
      Alternate Names
      • Cochlin
      • COCH-5B2
      Background InformationThe protein Cochlin/COCH is important in the development of the cochlea and vestibule in mammals as well as other functions. Cochlin/COCH is an extracellular matrix protein that plays a role in cell shape, positioning and motility. Recent research shows that it is also important in our innate immune response to bacteria and pathogens. Upon bacterial infection, a fragment of Cochlin/COCH is released from the conduits of B cell follicles in the spleen and this triggers protective cytokines in the periphery. Cochlin/COCH is expressed in epithelial tissues in various tissues, particularly in follicular cells and network of conduits in the spleen and lymph nodes. Cochlin/COCH also is a target for BMP4 mediation in embryonic stem cells (ESCs) where it facilitates self-renewal. The protein also appears to suppress neural differentiation of ESCs and suggests that Cochlin/COCH protein is important in ES cell fate determination. Mutations in the Cochlin/COCH gene are responsible for human DFNA9 syndrome, a disorder characterized by neurodegeneration of the inner ear that leads to hearing loss and vestibular impairments.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cochlin Antibody/COCH, clone 9A10D2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cochlin/COCH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Cochlin/COCH in 10 µg of human lymph node tissue lysate.
      Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
      Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
      Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Cochlin/COCH.
      Clone9A10D2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COCH
      • COCH5B2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~59 kDa observed. This protein is cleaved into p18 and p8 products. Uncharacterized bands may be observed in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse spleen tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABF267 04055977112696