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MABE969 Anti-VprBP Antibody, clone 8A7.2

Anti-VprBP Antibody, clone 8A7.2 is an antibody against VprBP for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-VprBP Antibody, clone 8A7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: VPRBP, DCAF1, KIAA0800, RIP UniProt Number: Q9Y4B6
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MABE969
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)MProtein G PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE969
      DescriptionAnti-VprBP Antibody, clone 8A7.2
      Alternate Names
      • Protein VPRBP;DDB1- and CUL4-associated factor 1
      • HIV-1 Vpr-binding protein
      • Serine/threonine-protein kinase VPRBP
      • Vpr-interacting protein
      • VprBP
      Background InformationProtein VPRBP (EC 2.7.11.1; UniProt Q9Y4B6; also known as DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP) is encoded by the VPRBP (also known as DCAF1, KIAA0800, RIP) gene (Gene ID 9730) in human. VprBP/DCAF1 acts as an adaptor molecule that mediates substrates recruitment for both the RING-type CRL4 (Cul4-Roc1-DDB1) and HECT-type EDD/UBR5 E3 ubiquitin ligase complexes. CRL4 substrates known to be recruited by VprBP include Merlin, Mcm10, UNG2, SMUG1, RORαme1. Known EDD/UBR5 substrates recruited by VprBP include Katanin and TERT. VprBP is involved in a variety of normal cellular processes, including proliferation, DNA replication, cell cycle progression, telomere maintenance, and DNA damage responses. As indicated by its name, VprBP is also a well known target utilized by the Vpr and Vpx accessory factors of primate lentiviruses to highjack the host CRL4VprBP E3 ligase function.
      References
      Product Information
      FormatProtein G Purified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-VprBP Antibody, clone 8A7.2 is an antibody against VprBP for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected VprBP in human kidney, human small intestine, and rat testis tissue sections.
      Biological Information
      ImmunogenBCP-conjugated linear peptide corresponding an N-terminal sequence of human VprBP.
      EpitopeN-terminal region.
      Clone8A7.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all three spliced isoforms of human VprBP reported by UniProt (Q9Y4B6).
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VPRBP
      • DCAF1
      • KIAA0800
      • RIP
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~169 kDa observed. 169.0 kDa (isoform 1), 168.9 kDa (isoform 2), and 118.2 kDa (isoform 3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected VprBP in 10 µg of HCT116 human colon carcinoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE969 04055977331233