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684274 β1,2-Xylosidase, Xanthomonas sp.

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684274
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684274-500MIU
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      Description
      OverviewNative β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
      Note: 1 mU = 1 milliunit.
      Catalogue Number684274
      Brand Family Calbiochem®
      Synonymsβ-D-Xylanxylohydrolase
      References
      ReferencesReese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.
      Product Information
      CAS number9025-53-0
      Activity≥10 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
      EC number3.2.1.37
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥20 units/mg protein
      Physicochemical Information
      Contaminantsβ-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsFollowing initial use, refrigerate (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      684274-500MIU 04055977225358

      Documentation

      β1,2-Xylosidase, Xanthomonas sp. SDS

      Title

      Safety Data Sheet (SDS) 

      β1,2-Xylosidase, Xanthomonas sp. Certificates of Analysis

      TitleLot Number
      684274

      References

      Reference overview
      Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      Synonymsβ-D-Xylanxylohydrolase
      DescriptionNative β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditionsRecommended reaction buffer: 50 mM sodium acetate buffer, 5 mM CaCl2, pH 5.0.
      CAS number9025-53-0
      EC number3.2.1.37
      Contaminantsβ-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases: none detected
      Specific activity≥20 units/mg protein
      Activity≥10 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsFollowing initial use, refrigerate (4°C).
      Toxicity Standard Handling
      ReferencesReese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.