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525320 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

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525320
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

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525320-100UG
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      Description
      OverviewPeroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
      Catalogue Number525320
      Brand Family Calbiochem®
      References
      ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
      Preservative0.1% Proclin
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (1:2000)
      Immunoblotting (1:500-1:2000)
      Immunohistochemistry (1:500)
      Application CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
      ClonePY20
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      525320-100UG 07790788051129