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MABE61 Anti-macro-H2A1.2 Antibody, clone 14G7

Anti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC.

Anti-macro-H2A1.2 Antibody, clone 14G7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1, MACROH2A1.1, mH2A1, macroH2A1.2, macro-H2A1.2 UniProt Number: O75367
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MABE61
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE61
      DescriptionAnti-macro-H2A1.2 Antibody, clone 14G7
      Alternate Names
      • H2A histone family, member Y
      • Histone macroH2A1
      • Medulloblastoma antigen MU-MB-50.205
      • histone macroH2A1.1
      • histone macroH2A1.2
      • Core histone macro-H2A.1
      Background InformationHistone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant associated with condensed, inactive heterochromatin. This histone variant consists of a histone H2A-like domain fused to a large non-histone region. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. The non-histone region of mH2A is directly involved in the repression of transcription, histone acetylation and blocking SWI/SNF and ACF nucleosome remodeling. This variant histone is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci.
      References
      Product Information
      FormatPurified
      Control
      • MCF-7 cell lysate
      PresentationPurified mouse monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human macro-H2A1.2.
      Clone14G7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
      Gene Symbol
      • H2A.y
      • H2A/y
      • H2AF12M
      • H2AFJ
      • MACROH2A1
      • MACROH2A1.1
      • mH2A1
      • macroH2A1.2
      • macro-H2A1.2
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).

      SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. TISSUE SPECIFICTY: Ubiquitous.

      PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.

      MISCELLANEOUS: The number of individuals with antibodies against this antigen is 2-fold higher in pediatric patients with cancer compared to healthy controls.

      SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.

      Contains 1 Macro domain.
      Molecular Weight~39 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected macro-H2A1.2 on 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE61 04053252292248

      Documentation

      Anti-macro-H2A1.2 Antibody, clone 14G7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-macro-H2A1.2 Antibody, clone 14G7 Certificates of Analysis

      TitleLot Number
      Anti-macro-H2A1.2, clone 14G7 - 2137016 2137016
      Anti-macro-H2A1.2, clone 14G7 - 2167757 2167757
      Anti-macro-H2A1.2, clone 14G7 - 2301687 2301687
      Anti-macro-H2A1.2, clone 14G7 - 3179933 3179933
      Anti-macro-H2A1.2, clone 14G7 - 3270775 3270775
      Anti-macro-H2A1.2, clone 14G7 - 3782283 3782283
      Anti-macro-H2A1.2, clone 14G7 - 3911115 3911115
      Anti-macro-H2A1.2, clone 14G7 - 4050749 4050749
      Anti-macro-H2A1.2, clone 14G7 - 4116912 4116912
      Anti-macro-H2A1.2, clone 14G7 - 4150403 4150403
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