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MABE362 Anti-EZH2 Antibody, clone AE25

This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.

Anti-EZH2 Antibody, clone AE25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: ENX-1, KMT6 UniProt Number: Q15910
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MABE362
100 µL  
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      Overview

      Replacement Information

      Special Offers

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE362
      Replaces04-1047
      DescriptionAnti-EZH2 Antibody, clone AE25
      Alternate Names
      • Enhancer of zeste homolog 2
      • Lysine N-methyltransferase 6
      • enhancer of zeste 2
      • histone-lysine N-methyltransferase EZH2
      Background InformationEZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
      References
      Product Information
      FormatPurified
      Control
      • T47D cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.

      Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
      Biological Information
      ImmunogenMBP-tagged recombinant protein corresponding to human EZH2.
      CloneAE25
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ENX-1
      • KMT6
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80 kDa observed. Uniprot describes multiple isoforms ranging from 80-86 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in T47D cell lysate.

      Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE362 04053252551819

      Documentation

      Anti-EZH2 Antibody, clone AE25 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EZH2 Antibody, clone AE25 Certificates of Analysis

      TitleLot Number
      Anti-EZH2, clone AE25 - 2458016 2458016
      Anti-EZH2, clone AE25 - 3472441 3472441
      Anti-EZH2, clone AE25 - 3698189 3698189
      Anti-EZH2, clone AE25 - 4154506 4154506
      Anti-EZH2, clone AE25 - Q2062626 Q2062626
      Anti-EZH2, clone AE25 -2572523 2572523
      Anti-EZH2, clone AE25 -2621117 2621117
      Anti-EZH2, clone AE25 Monoclonal Antibody 3048308
      Anti-EZH2, clone AE25 Monoclonal Antibody 2902715

      References

      Reference overviewPub Med ID
      EZH2 and BMI1 inversely correlate with prognosis and TP53 mutation in breast cancer.
      Pietersen, Alexandra M, et al.
      Breast Cancer Res., 10: R109 (2008)  2008

      Show Abstract       		19099573 19099573

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies