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MABD42 Anti-Cyp7a1 Antibody, clone 15B9.1

This Cyp7a1 antibody is validated for use in WB & IHC for the detection of the Cyp7a1 protein.

Anti-Cyp7a1 Antibody, clone 15B9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CYP7A1, CYP7 UniProt Number: P22680
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MABD42
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABD42
      DescriptionAnti-Cyp7a1 Antibody, clone 15B9.1
      Alternate Names
      • Cholesterol 7-alpha-monooxygenase
      • CYPVII
      • Cholesterol 7-alpha-hydroxylase
      • Cytochrome P450 7A1
      Background InformationCytochrome P450 7A1 (CYP7A1, CYPVII), also known as cholesterol 7-alpha-monooxygenase or cholesterol 7-alpha-hydroxylase, belongs to the cytochrome P450 family and is important for cholesterol homeostasis. CYP7A1 catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Detected primarily in the liver, CYP7A1 catalyzes the following reaction: Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O. CYP7A1 is up-regulated by glucose and by cholestyramine, and is down-regulated by chenodeoxycholic acid.
      References
      Product Information
      FormatPurified
      Control
      • Human liver tissue lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Cyp7a1 antibody is validated for use in WB & IHC for the detection of the Cyp7a1 protein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImunnohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Cyp7a1 in rat and human liver tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Cyp7a1.
      Clone15B9.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYP7A1
      • CYP7
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~52 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human liver tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cyp7a1 in 10 µg of human liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD42 04053252854279

      Documentation

      Anti-Cyp7a1 Antibody, clone 15B9.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cyp7a1 Antibody, clone 15B9.1 Certificates of Analysis

      TitleLot Number
      Anti-Cyp7a1, clone 15B9.1 2475858
      Anti-Cyp7a1, clone 15B9.1 Monoclonal Antibody 2949965
      Anti-Cyp7a1, clone 15B9.1 - Q2129760 Q2129760
      Anti-Cyp7a1, clone 15B9.1 - 3173803 3173803
      Anti-Cyp7a1, clone 15B9.1 - 3211121 3211121
      Anti-Cyp7a1, clone 15B9.1 - 3328986 3328986
      Anti-Cyp7a1, clone 15B9.1 - 3604327 3604327
      Anti-Cyp7a1, clone 15B9.1 - 3978837 3978837
      Anti-Cyp7a1, clone 15B9.1 -2586831 2586831
      Anti-Cyp7a1, clone 15B9.1 -2649283 2649283
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