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07-896-I Anti-phospho-Rac1/cdc42 (Ser71) Antibody

Detect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.

Anti-phospho-Rac1/cdc42 (Ser71) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000
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07-896-I
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-896-I
      DescriptionAnti-phospho-Rac1/cdc42 (Ser71) Antibody
      Alternate Names
      • Ras-related C3 botulinum toxin substrate 1
      • Cell migration-inducing gene 5 protein
      • Ras-like protein TC25
      • p21-Rac1
      • phospho-Rac1/cdc42 (Ser71)
      Background InformationTarget of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 2.5µg from a representative lot immunoprecipitated phospho-Rac1/cdc42 (Ser71) in EGF treated A431 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Rac1/cdc42 (Ser71) near the N-Terminus.
      EpitopeNear N-Terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Rhesus Macaque, Xenopus, Zebrafish, Chicken , Orangatan based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAC1
      • TC25
      • MIG5
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~25 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in EGF treated A431 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rac1/cdc42 (Ser71) in 10 µg of EGF treated A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-896-I 04055977176438

      Documentation

      Anti-phospho-Rac1/cdc42 (Ser71) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Rac1/cdc42 (Ser71) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-Rac1/cdc42 (Ser71) - 3389918 3389918
      Anti-phospho-Rac1/cdc42 (Ser71) - 3934291 3934291
      Anti-phospho-Rac1/cdc42 (Ser71) - 4082547 4082547
      Anti-phospho-Rac1/cdc42 (Ser71) -Q2581246 Q2581246

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies