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05-503-I Anti-LacI Antibody, clone 9A5

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05-503-I
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      E. coliWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-503-I
      DescriptionAnti-LacI Antibody, clone 9A5
      Alternate Names
      • Lactose operon repressor
      Background InformationThe lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.
      References
      Product Information
      FormatPurified
      Control
      • Rosetta BL21 (DE3) competent E. Coli cell lysate.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).
      Quality LevelMQ100
      Applications
      ApplicationAnti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenTrpE fused with full-length E. Coli LacI.
      Clone9A5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • E. coli
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • lacI
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~38 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Rosetta™ BL21 (DE3) E. Coli cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-503-I 04053252819209

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies