Millipore Sigma Vibrant Logo

07-748-AF488 Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4 UniProt Number: P62805
View Products on Sigmaaldrich.com
07-748-AF488
100 µL  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number07-748-AF488
      DescriptionAnti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate
      Alternate Names
      • H4K20me1
      • Histone H4, Lys20 monomethylated
      • H4 histone family member, Lys20 monomethylated
      Background InformationHistone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H4 Lys20 monomethylation in HeLa cells.
      The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.
      Biological Information
      ImmunogenKLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[Kme]VLRDN) of human Histone H4 with monomethylated Lys20.
      EpitopeH4K20me1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects histone H4 with monomethylated Lys20 (H4K20me1).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Broad species cross-reactivity expected due to sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~10 kDa observed.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      07-748-AF488 04055977350647