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MABS1235 Anti-Progesterone Receptor A/B Antibody, clone 488/H3

This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PGR, NR3C3 UniProt Number: P06401
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MABS1235
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1235
      DescriptionAnti-Progesterone Receptor A/B Antibody, clone 488/H3
      Alternate Names
      • Progesterone receptor
      • PR
      • Nuclear receptor subfamily 3 group C member 3
      • Progesterone Receptor A/B
      Background InformationProgesterone receptor (UniProt P06401; also known as nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B, but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected exogenously expressed progesterone receptor isoform A and isoform B (PR-A & PR-B) in transfected MCF10A cells by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
      Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoforms A & B (PR-A & PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-A & PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminal half fragment of human Progesterone Receptor B.
      EpitopeInternal
      Clone488/H3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 488/H3 reacts with both isoform 1 (isoform B; PR-B) and isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PGR
      • NR3C3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110/80 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human breast cancer T47D cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected both isoforms A and B (PR-A & PR-B) of progesterone receptor in human breast cancer T47D cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1235 04055977288407