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07-745-I Anti-phospho Histone H2A (Ser129) Antibody

Detect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A (Ser129) Antibody validated for use in western blotting, Dot Blot, Peptide Inhibition Assay, IP & ICC.

Anti-phospho Histone H2A (Ser129) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HTA1, H2A1, SPT11 UniProt Number: P04911
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07-745-I
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      YeastWB, DB, PIA, IP, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-745-I
      DescriptionAnti-phospho Histone H2A (Ser129) Antibody
      Alternate Names
      • Histone H2A.1
      Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A (Ser129) Antibody validated for use in western blotting, Dot Blot, Peptide Inhibition Assay, IP & ICC.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Peptide Inhibition Assay
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesDot Blot (Specificity) Analysis: 0.05 µg/mL from a representative lot detected phospho Histone H2A (Ser129) in various panels of modified and non-modified Histones.

      Immunoprecipitation Analysis: A 1:100 dilution from a represenetative lot immunoprecipitated phospho Histone H2A (Ser129) from yeast extracts.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a lot detected phospho Histone H2A (Ser129) from yeast cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of yeast Histone H2A phosphorylated at Ser129.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody is specifically recognizes yeast phospho-Histone H2A (Ser129)
      Species Reactivity
      • Yeast
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HTA1
      • H2A1
      • SPT11
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and Methyl methanesulfonate (MMS) treated yeast nuclear extract.

      Western Blotting Analysis: 0.02 µg/mL of this antibody detected an increase in signal for phospho Histone H2A (Ser129) in MMS treated yeast nuclear extract over untreated yeast nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-745-I 04053252935893

      Documentation

      Anti-phospho Histone H2A (Ser129) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho Histone H2A (Ser129) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho Histone H2A (Ser129) 2485988
      Anti-phospho Histone H2A (Ser129) - 3308254 3308254
      Anti-phospho Histone H2A (Ser129) - 3671316 3671316
      Anti-phospho Histone H2A (Ser129) - 4013894 4013894
      Anti-phospho Histone H2A (Ser129) - Q2315818 Q2315818
      Anti-phospho Histone H2A (Ser129) -2764889 2764889
      Anti-phospho Histone H2A (Ser129) Polyclonal Antibody 3147029
      Anti-phospho Histone H2A (Ser129) Polyclonal Antibody 3054707

      Brochure

      Title
      New Products Flyer- Feb 2014 - Vol 1