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ABF1207 Anti-phospho-Syk (Tyr323)

This rabbit polyclonal Anti-phospho-Syk (Tyr323) antibody, Cat. No. ABF1207 is validated for use in Western Blotting for the detection of Syk Tyr323 phosphorylation.

Anti-phospho-Syk (Tyr323) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Inflammation & Immunology Gene Symbol: SYK UniProt Number: P43405
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ABF1207
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF1207
      Replaces07-915
      DescriptionAnti-phospho-Syk (Tyr323)
      Alternate Names
      • Tyrosine-protein kinase Syk, Tyr323 phosphorylated
      • p72-Syk, Tyr323 phosphorylated
      • p72Syk, Tyr323 phosphorylated
      • Spleen tyrosine kinase, Tyr323 phosphorylated
      Background InformationTyrosine-protein kinase Syk (EC 2.7.10.2; UniProt P43405; also known as p72-Syk, p72Syk, Spleen tyrosine kinase) is encoded by the SYK gene (Gene ID 6850) in human. Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase highly expressed by haematopoietic cells. Syk plays a crucial role not only in adaptive immune receptor signalling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk adopts an autoinhibited conformation in its inactive state and is activated upon binding immunoreceptor tyrosine-based activation motifs (ITAMs) and phosphorylation of tyrosine residues in the two linker regions (a.a. 108-160 and 260-370) between SH1 (a.a. 15-107) and SH2 (a.a. 168-259), and between SH2 and the kinase domain (a.a. 371-631). Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. In additon, Syk can phosphorylate ITAMs and autophosphorylate its own link region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk also mediates signalling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR γ-chain (FcRγ).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-phospho-Syk (Tyr323) antibody, Cat. No. ABF1207 is validated for use in Western Blotting for the detection of Syk Tyr323 phosphorylation.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.
      EpitopepTyr323
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYK
      Modifications
      • Phosphorylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~72/69 kDa observed. 72.07/69.51 kDa (human Syk isoform 1/2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Syk Tyr323 phosphorylation induction in 10 µg of lysate from hydrogen peroxide-treated Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABF1207 04054839069642

      Documentation

      Anti-phospho-Syk (Tyr323) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-phospho-Syk (Tyr323) Certificats d'analyse

      TitreNuméro de lot
      Anti-phospho-Syk (Tyr323) - 3492217 3492217
      Anti-phospho-Syk (Tyr323) -2890159 2890159
      Anti-phospho-Syk (Tyr323) -Q2753916 Q2753916
      Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2919543
      Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2955679
      Anti-phospho-Syk (Tyr323) Polyclonal Antibody 2863774
      Anti-phospho-Syk (Tyr323) Polyclonal Antibody_2837113 2837113
      Anti-phospho-Syk (Tyr323), Polyclonal Antibody 2832824