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OP32 Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246)

This Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) is validated for use in Immunocytochemistry, Paraffin Sections, Immunoblotting for the detection of p53 (Ab-4) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP32
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in SV-T2 cells. Does not recognize mutant or denatured p53 protein.

      This product has been discontinued.
      Catalogue NumberOP32
      Brand Family Calbiochem®
      Application Data
      Detection of mouse p53 by immunocytochemical staining. Sample: SV-T2 cells fixed in methanol. Primary antibody: Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) (Cat. No. OP32) (1 µg/ml). Detection: fluorescence with DAPI counterstain.
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlSK-OV-3 cells
      Positive controlSV-T2 cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesOriginal Clone Yewdell, J.W., et al. 1986. J. Virol. 59, 444.
      Key Applications Immunocytochemistry
      Immunoprecipitation
      Not Western Blot
      Paraffin Sections
      Application NotesImmunocytochemistry (1-20 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
      Immunoblotting (not recommended)
      Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met (less than 1 h) will help reduce background. Anti-p53 (Ab-4) (Wild type), preferentially recognizes wild-type p53 of murine cellular origin; it will not react with mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenBALB/c SVA31 E7 cells
      ImmunogenMouse
      Epitopea conformational epitope within amino acids 85-109
      ClonePAb246
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP32 0

      Documentation

      Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Certificates of Analysis

      TitleLot Number
      OP32

      References

      Reference overview
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunocytochemistry (1-20 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
      Immunoblotting (not recommended)
      Application Data
      Detection of mouse p53 by immunocytochemical staining. Sample: SV-T2 cells fixed in methanol. Primary antibody: Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) (Cat. No. OP32) (1 µg/ml). Detection: fluorescence with DAPI counterstain.
      DescriptionPurified mouse monoclonal antibody generated by immunizing C57/BL mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type p53 protein.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
      HostMouse
      Immunogen speciesMouse
      ImmunogenBALB/c SVA31 E7 cells
      Epitopea conformational epitope within amino acids 85-109
      ClonePAb246
      IsotypeIgG₁
      Speciesnot human, mouse, rat
      Positive controlSV-T2 cells
      Negative controlSK-OV-3 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met (less than 1 h) will help reduce background. Anti-p53 (Ab-4) (Wild type), preferentially recognizes wild-type p53 of murine cellular origin; it will not react with mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Application referencesOriginal Clone Yewdell, J.W., et al. 1986. J. Virol. 59, 444.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies