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SCC293 U2OS EGFP-H2B Human Osteosarcoma Cell Line

U2OS EGFP-H2B Human Osteosarcoma Cell Line allows for visualization of chromosome dynamics by light microscopy

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SCC293
≥1X10⁶ cells/vial   
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      Overview

      Description
      Catalogue NumberSCC293
      DescriptionU2OS EGFP-H2B Human Osteosarcoma Cell Line
      OverviewChromosomal abnormalities underlie a substantial proportion of cancers, including most solid tumors (1). Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrange
      Alternate Names
      • U-2 OS EGFP-H2B Human Osteosarcoma Cell Line
      • U-2OS EGFP-H2B Human Osteosarcoma Cell Line
      • U-2-OS EGFP-H2B Human Osteosarcoma Cell Line
      • U2-OS EGFP-H2B Human Osteosarcoma Cell Line
      • U20-S EGFP-H2B Human Osteosarcoma Cell Line
      • U20S EGFP-H2B Human Osteosarcoma Cell Line
      • 2T EGFP-H2B Human Osteosarcoma Cell Line
      Background InformationChromosomal abnormalities underlie a substantial proportion of cancers, including the majority of solid tumors.1 Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrangements.1 Understanding the mechanisms of chromosomal instability is key to unlocking the multifaceted roles that they play in cancer etiology and progression.
      The U2OS osteosarcoma is one of the first and most widely used cancer cell lines, especially for the study of mitosis, apoptosis, and the cellular mechanisms that give rise to cancer. U2OS cells undergo chromosome breakage and chromothripsis at high frequency.2 The expression of EGFP fused to a histone marker in the U2OS EGFP-H2B cell line allows for visualization of chromosome dynamics by light microscopy, a powerful tool in unraveling the complex interactions and molecular players involved in chromosomal instability.3 Expression of EGFP-H2B in this cell line is maintained via selection with blasticidin. The U2OS EGFP-H2B cell line facilitates expanded insights to chromosomal biology, allowing immediate relevancy to the many studies using the well-characterized U2OS background.

      Source
      The U2OS EGFP-H2B Human Osteosarcoma cell line was derived from U2OS cells stably transfected with a construct expressing EGFP-histone-H2B.3 The parental U2OS cell line was derived from an osteosarcoma from a 15-year-old female.2

      References
      1. Thompson SL, Compton DA. Chormosome Res. 2011; 19(3):433-444.
      2. Pontén J, Saksela E. Int J Cancer. 1967; 2(5):434-447.
      3. Wang H et al. Oncotarget 2017; 8(30):48671-48687.
      Applications
      ApplicationU2OS EGFP-H2B Human Osteosarcoma Cell Line allows for visualization of chromosome dynamics by light microscopy
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Product Usage Statements
      Quality AssuranceEach vial contains ≥ 1X10⁶ viable cells.
      Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Global Trade Item Number
      Catalogue Number GTIN
      SCC293 04065266024159