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DR1052 Anti-SALL4 Mouse mAb (6E3)

This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Anti-SALL4 Mouse mAb (6E3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SAL-Like 4

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DR1052
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

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DR1052-100UG
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      DR1052-2MG
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          Description
          OverviewRecognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
          Catalogue NumberDR1052
          Brand Family Calbiochem®
          SynonymsAnti-SAL-Like 4
          Application Data
          Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
          References
          ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
          Forte, A., et al. 2009. Mol. Med. 15 392.
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
          PreservativeNone
          Quality LevelMQ100
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.2 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
          ImmunogenNA
          Clone6E3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          DR1052-100UG 04055977225983
          DR1052-2MG 04055977225990

          Documentation

          Anti-SALL4 Mouse mAb (6E3) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-SALL4 Mouse mAb (6E3) Certificates of Analysis

          TitleLot Number
          DR1052

          References

          Reference overview
          Cauffman, G., et al. 2009. Hum. Reprod. 24 63.
          Forte, A., et al. 2009. Mol. Med. 15 392.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-August-2015 JSW
          SynonymsAnti-SAL-Like 4
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.2 µg/ml)
          Application Data
          Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
          BackgroundThis protein may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).
          HostMouse
          Immunogen speciesNA
          ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
          Clone6E3
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage Avoid freeze/thaw
          -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
          Forte, A., et al. 2009. Mol. Med. 15 392.