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FCMAB116P Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70

This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: SSEA-4, SSEA4
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FCMAB116P
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB116P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70
      OverviewN-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.
      Background InformationThe cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
      Product Information
      FormatPhycoerythrin
      Control
      • Pluripotent human embryonic stem (ES) cells
      PresentationPurified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
      Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
      Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
      Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
      Biological Information
      ImmunogenHuman embryonal carcinoma cell line 2102Ep.
      CloneMC-813-70
      HostMouse
      SpecificityThis antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
      No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
      IsotypeIgG3κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SSEA-4
      • SSEA4
      Purification MethodProtein A Purfied
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB116P 04053252371301