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MABF839 Anti-Ly-6G Antibody, clone 1A8 (Azide free)

MABF839
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, ACT, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF839
      DescriptionAnti-Ly-6G Antibody, clone 1A8 (Azide free)
      Alternate Names
      • Ly-6G
      • Lymphocyte antigen 6G
      Background InformationThe 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Ly-6G Antibody, clone 1A8 (Azide free) is an antibody against Ly-6G for use in Flow Cytometry, Activity Assay, Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Activity Assay
      • Immunoprecipitation
      Application NotesFlow Cytometry Analysis: 0.5 µg of this antibody from a representative lot detected Ly-6G in 1X10E6 mouse bone marrow cells.
      Flow Cytometry Analysis: A representative lot detected Ly-6G in transfected EL4 cells and bone marrow cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
      Activity Assay Analysis: A representative lot detected Ly-6G in transmission of S. pneumoniae from index after injection with this clone (Short, K.R., et al. (2013). mBio. 3(5):e00255-12).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Ly-6G in transfected EL4 cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
      Biological Information
      ImmunogenPropeitary whole cells expressing to mouse Ly-6G.
      Clone1A8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ly6g
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight12 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse splenocyte.

      Flow Cytometry Analysis: 0.5 µg of this antibody detected Ly-6G in mouse splenocyte.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF839 04055977151770

      Documentation

      Anti-Ly-6G Antibody, clone 1A8 (Azide free) Certificats d'analyse

      TitreNuméro de lot
      Anti-Ly-6G, clone 1A8 (Azide free) -Q2559139 Q2559139

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      The Journal of neuroscience : the official journal of the Society for Neuroscience
      Nelson, CD; Kim, MJ; Hsin, H; Chen, Y; Sheng, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  12122-35  2013

      Afficher le résumé
      23864697 23864697
      mBio
      Short, KR; Reading, PC; Wang, N; Diavatopoulos, DA; Wijburg, OL
      mBio  3  2011

      Afficher le résumé
      23015738 23015738

      Brochure

      Titre
      NPI Flyer- Epigenetics and Nuclear Function Feature

      Informations techniques

      Titre
      White Paper: Further considerations of antibody validation and usage.