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07-473-AF555 Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate

This Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FB, HIST1H3C, H3FC, HIST1H3D, H3FD, HIST1H3E, HIST1H3F, H3FF, HIST1H3G, HIST1H3H, H3FH, HIST1H3I, H3FI, HIST1H3J, H3FJ, H3FK, HIST3H3, H3FT, H3T UniProt Number: Q16695
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07-473-AF555
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®555Polyclonal Antibody
      Description
      Catalogue Number07-473-AF555
      DescriptionAnti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate
      Alternate Names
      • H3K4me3
      • H3.3B, Lys4 trimethylated
      • H3 histone, family 3A, Lys4 trimethylated
      • H3 histone, family 3B, Lys4 trimethylated
      • Histone H3, Lys4 trimethylated
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected detected histone H3 Lys4 trimethylation in HeLa cells.
      The unconjugated antibody (Cat. No. 07-473) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, and Western blotting applications.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding and including trimethlyated Lys4 of histone H3.
      EpitopeTrimethylated Lys4 (H3K4me3).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody only detects histone H3 when trimethylated on Lys4.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Expected to react with most other vertebrate species based on highly conserved sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FB
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FD
      • HIST1H3E
      • HIST1H3F
      • H3FF
      • HIST1H3G
      • HIST1H3H
      • H3FH
      • HIST1H3I
      • H3FI
      • HIST1H3J
      • H3FJ
      • H3FK
      • HIST3H3
      • H3FT
      • H3T
      Modifications
      • Methylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~16 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys4 trimethylation in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      07-473-AF555 04055977351446

      Documentation

      Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate Certificados de análisis

      CargoNúmero de lote
      Anti-trimethyl Histone H3 (Lys4), -Q2688174 Q2688174
      Anti-trimethyl Histone H3 (Lys4), Alexa Fluor® 555 Conjugate - 3379905 3379905
      Anti-trimethyl Histone H3 (Lys4), Alexa Fluor® 555 Conjugate - 4072173 4072173
      Anti-trimethyl Histone H3 (Lys4), Alexa Fluor® 555 Conjugate Polyclonal Antibody 3002766