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MABE941 Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody

This Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ
UniProt Number: P68431
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MABE941
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DB, ICC, ELISA, ChIP, ChIP-seqRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE941
      ReplacesMABE13
      DescriptionAnti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/I
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunocytochemistry
      • ELISA
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

      ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

      Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides.

      Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013)
      ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho Histone H3.1 (Ser28).
      Clone5D10D4
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in a nocodozole treated HeLa cell lysate.

      Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABE941 04055977112139

      Documentation

      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody Certificados de análisis

      CargoNúmero de lote
      Anti-phospho Histone H3.1 (Ser28), -2776225 2776225
      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3071357 3071357
      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3460497 3460497
      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3860855 3860855
      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 4181439 4181439
      Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Monoclonal Antibody Q2475128

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
      Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T
      Monoclonal antibodies in immunodiagnosis and immunotherapy  32  119-24  2013

      Mostrar resumen       		23607347 23607347