SNAP i.d.® 2.0 Protein Detection System
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Overview
Developed to meet the needs of our Western blotting customers, the SNAP i.d.® 2.0 system produces blots of a very high quality every time – in record time! Unique vacuum-driven technology and a built-in flow distributor actively drive reagents through the membrane, ensuring even distribution. Two blot holder sizes accommodates mini Mini (7.5 x 8.4 cm) or midi Midi (8.5 x 13.5 cm) (W x L) ) size gels and two blot holders can be run in parallel. Thus, you can quickly optimize conditions and greatly increase your protein detection throughput.
Typically, researchers lack the time to optimize their blotting protocols. By shortening the time required for blocking, washing and antibody incubations to 30 minutes, the SNAP i.d.® 2.0 system allows you to optimize your immunodetection conditions for the highest quality results.
The ordering tables on the next tab show how you can easily obtain the SNAP i.d.® 2.0 system and its components. We look forward to hearing about your research results once you have used your new tool for Western blotting!
SNAP i.d.® 2.0 Hardware and Accessories
SNAP i.d.® 2.0 Mini Blot Holder
SNAP i.d.® 2.0 Midi Blot Holder
SNAP i.d.® 2.0 Antibody Collection Tray
SNAP i.d.® 2.0 Blot Roller
Your SNAP i.d.® 2.0 system includes everything you need to get started, including blot holders, two independent blot holder frames, antibody collection tray and blot roller. In addition, we provide you with an integrated vacuum regulator to ensure consistent vacuum pressure without the need for an external regulator.
Other useful items include: Chemical duty pumps (115 V/60 Hz) or (220 V/50 Hz), a one-liter Vacuum filtering flask, Silicone No. 8 perforated stoppers (5 to a pack) and stainless steel forceps.
Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d.® 2.0 system uses a vacuum to actively drive reagents through the membrane, enabling thorough washing and reducing the blocking, antibody incubation and wash steps to 30 minutes total. The SNAP i.d.® 2.0 system adds exciting new capabilities for Western blotting and requires no additional reagent consumption (e.g., antigen, antibody or detection reagents). The system’s unique design enables the use of small volumes for antibody incubations with either polyvinylidene difluoride (PVDF) or nitrocellulose blotting membranes.
Features & Benefits
- Superior: Increased antibody-antigen binding, enhanced washes, and antibody recollection
- Flexible: Two gel sizes, mini (7.5 x 8.4 cm) and midi (8.7 x 13.5 cm)
- Fast: 30-minute immunodetection
Applications
Western Blotting using Nitrocellulose or PVDF Membrane; Compatible with Radioactive, Chromogenic, Chemiluminescent, Fluorescent, and Chemifluorescent Detection