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Buffer Detection Kit for Magnetic Beads
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480010
MilliporeNDSB-256 - CAS 81239-45-4 - Calbiochem
A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.
More>>A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Less<<
NDSB-256 - CAS 81239-45-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.
Catalogue Number
480010
Brand Family
Calbiochem®
Synonyms
Dimethylbenzylammonium Propane Sulfonate
References
References
Goldberg, M.E., et al. 1996. Folding & Design1, 21.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-April-2012 JSW
Synonyms
Dimethylbenzylammonium Propane Sulfonate
Description
A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.
Form
White solid
CAS number
81239-45-4
Chemical formula
C₁₂H₁₉NO₃S
Structure formula
Purity
≥99% by TLC
Solubility
H₂O (200 mg/ml)
Storage
+15°C to +30°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.
Toxicity
Irritant
References
Goldberg, M.E., et al. 1996. Folding & Design1, 21.
