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FCMAB214P Milli-Mark™ Anti-CD117 Antibody, c-kit-PE, clone 104D2

Milli-Mark Anti-CD117 Antibody, c-kit-PE, clone 104D2 detects level of CD117 & has been published & validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P10721
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FCMAB214P
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB214P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD117 Antibody, c-kit-PE, clone 104D2
      Alternate Names
      • Mast/stem cell growth factor receptor
      • SCFR
      • Tyrosine-protein kinase Kit
      • Proto-oncogene c-Kit
      Background InformationCD117 is a 145 kDa transmembrane protein which belongs to the class III receptor tyrosine kinase family. The extracellular region consists of five immunoglobulin-like domains where the second and third loops are thought to be involved in ligand binding (Ashman, 1997; Bühring, 1995). CD117 has been shown to be a marker for tissue mast cells, hematopoietic stem cells, and progenitor cells in normal human bone marrow. The majority of CD117-positive marrow cells (50-70%) co-express CD34 and comprise progenitor cells and their precursors of all hematopoietic lineages. Apart from mast cells, CD117 is essentially absent from mature hematopoietic cells. Although CD117 is primarily a marker for non-lymphoid progenitor cells in the hematopoietic cells/tissues, including melanocytes, embryonic brain, and the reproductive system (Ashman, 1997). Anti-CD117, 104D2, was included in the Sixth International Workshop and Conference on Human Leucocyte differentiation Antigens, and studies by different laboratories confirmed its reactivity with CD117 (Ashman, 1997).
      Product Information
      FormatPhycoerythrin
      Control
      • TF-1 cells
      PresentationPurified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationMilli-Mark Anti-CD117 Antibody, c-kit-PE, clone 104D2 detects level of CD117 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenMOLM-1 megakaryocytic cell line.
      Clone104D2
      HostMouse
      SpecificityAntibody recognizes Human CD117 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryThis is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (PI3K).

      Defects in KIT are a cause of piebaldism. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes
      Molecular Weight145 kDa (Ashman, 1997; Buhring, 1995)
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using TF-1 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB214P 04053252480232