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F0815-BC Ham's F-12 Liquid Medium without L-Glutamine

with 1.176 g/l NaHCO₃, with 10 mg/l phenol red

Ham's F-12 Liquid Medium without L-Glutamine MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
F0815-BC
  
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      Overview

      Replacement Information
      Description
      OverviewNutrient mixture Ham's F-10 and F-12 were optimized for the serum free growth of hamster ovary strains and the clonal growth of chick embryo cells. Ham's F-10 is well suited for a wide variety of mammalian cells when used with a serum supplement in a 5% CO2 atmosphere. Ham's F-12 has higher levels of amino acids, vitamins and trace elements vs. Ham's F-10. Putrescine and linoleic acid were added to the formulation. Ham's F-12 today is the medium of choice for clonal toxicity-assays using CHO cells (CHD-3 and CHL-1).
      Specifications:

      Sterility: Pass
      BHK 21 Cell Proliferation Test: Pass
      BHK 21 Cell Cloning Efficicency Test: Pass
      pH: 7.1-7.4
      Osmolality: 0.28-0.31 Osm/kg H20
      Endotoxin: < 0.5 EU/ml
      Color: Red
      Appearance: Clear
      Catalogue NumberF0815-BC
      Brand Family Calbiochem®
      References
      ReferencesHam. R.G. 1965. Proc. Nat. Acad. Sci. 53, 288.
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      F0815-BC 0

      Documentation

      References

      Reference overview
      Ham. R.G. 1965. Proc. Nat. Acad. Sci. 53, 288.