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CBA059 Annexin V-FITC Apoptosis Detection Kit II

This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells.

Annexin V-FITC Apoptosis Detection Kit II MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CBA059
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Overview

Key Spec Table

Detection Methods
Fluorescence

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      Description
      OverviewA convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
      Catalogue NumberCBA059
      Brand Family Calbiochem®
      Materials Required but Not Delivered• 1X PBS
      • Flow cytometer or fluorescence microscope
      References
      ReferencesDarzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
      Frey, T. 1997. Cytometry 28, 253;
      Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
      White, E. 1996. Genes and Devel. 10, 1.
      Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
      Koopman, G., et al. 1994. Blood 84, 1415.
      Wyllie, A.H.,1993. Br. J. Cancer 67, 205.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
      Schmid, I., et al. 1992. Cytometry 13, 204.
      Kerr, J.F.R., et al. 1972. Cancer 26, 239.
      Product Information
      Detection methodFluorescence
      Form20 Tests
      FormatFlow cytometry or fluorescence microscopy
      Kit containsAnnexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Immunofluorescence
      Biological Information
      Assay time30 min
      Sample TypeIntact cells
      Product Usage Statements
      Intended useThe Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the entire contents of the kit at 4°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsAnnexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      CBA059-1KIT 07790788053666