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SCC300 3dGRO™ Human iPSC Derived Colon Organoids

Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).

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SCC300
≥ 1500 organoids/vial  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC300
      Description3dGRO™ Human iPSC Derived Colon Organoids
      Alternate Names
      • Human intestinal organoids
      • Human colonic organoids
      • Human gut organoids
      Background InformationHuman intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).

      3dGRO™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO™ human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO™ Human Colon Organoid Expansion Medium (Cat. No. SCM304).
      References
      Product Information
      Applications
      ApplicationHighly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Cell Line Type
      • Organoid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1500 organoids.
      • Mycoplasma: Negative
      • Genotyped by STR analysis: Pass
      • Human species verified
      • Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
      • Cell viability test: Pass
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions3dGRO™ Human iPSC Derived Colon Organoids should be stored in liquid nitrogen.
      Packaging Information
      Material Size≥ 1500 organoids/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC300 04054839989728