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402176 ImmunoPen™

This ImmunoPen™ is validated for use in Frozen Sections, Immunostaining Barrier, Paraffin Sections.

ImmunoPen™ MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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402176
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Overview

Replacement Information

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402176-1EA
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      Description
      OverviewDesigned to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
      Catalogue Number402176
      Brand Family Calbiochem®
      References
      Product Information
      DeclarationNot available for sale in Japan.
      Applications
      Application NotesFrozen Sections
      Immunostaining Barrier
      Paraffin Sections
      Application CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

      Paraffin Section Method:
      Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
      Frozen Section Method:
      Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      402176-1EA 07790788054199

      Documentation

      ImmunoPen™ SDS

      Title

      Safety Data Sheet (SDS) 

      ImmunoPen™ Certificates of Analysis

      TitleLot Number
      402176

      Citations

      Title
    • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationFrozen Sections
      Immunostaining Barrier
      Paraffin Sections
      DescriptionDesigned to minimize antibody waste in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. The pen contains liquid resistant to water, alcohol, and acetone. When applied in a circle around the specimen the liquid forms a hydrophobic barrier, retaining antibody and minimizing waste of valuable reagents. Multiple sections, separated by individ-ual circular barriers, can be applied to the same slide. The ImmunoPen™ can be used for immunostaining procedures employing the following methods: PAP (peroxidase-antiperoxidase), ABC, enzymatic, and frozen section. While the barrier is insoluble in alcohol and acetone, it can be removed by xylene after the staining procedure is complete.
      CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

      Paraffin Section Method:
      Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
      Frozen Section Method:
      Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
      Toxicity Standard Handling
      Citation
    • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.
      		•