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MABS1188 Anti-PAFAH 1B3 Antibody, clone 3E4.1

Anti-PAFAH 1B3 Antibody, clone 3E4.1 is an antibody against PAFAH 1B3 for use in Western Blotting, Immunocytochemistry.

Anti-PAFAH 1B3 Antibody, clone 3E4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: PAFAH1B3, PAFAHG UniProt Number: Q15102
MABS1188
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1188
      DescriptionAnti-PAFAH 1B3 Antibody, clone 3E4.1
      Alternate Names
      • Platelet-activating factor acetylhydrolase IB subunit gamma
      • PAF acetylhydrolase 29 kDa subunit
      • PAF-AH 29 kDa subunit
      • PAF-AH1b subunit alpha 1
      • PAF-AH subunit gamma
      • PAFAH subunit gamma
      Background InformationPlatelet-activating factor acetylhydrolase IB subunit gamma (EC 3.1.1.47; UniProt Q15102; also known as PAF acetylhydrolase 29 kDa subunit, PAF-AH 29 kDa subunit, PAF-AH1b subunit alpha 1, PAF-AH subunit gamma, PAFAH subunit gamma) is encoded by the PAFAH1B3 (also known as PAFAHG) gene (Gene ID 5050) in human. Platelet-activating factor (PAF) acetylhydrolase (PAF-AH) negatively regulates PAF potency by catalyzing the removal of the acetyl group at the sn-2 position of the lipid messenger. There are two types of intracellular PAF-AH and a plasma type PAF-AH. Type I intracellular PAF-AH is a heterotrimeric G-protein-like complex composed of alpha1 (originally called gamma), alph2 (originally called alpha), and beta subunits. Both alpha1 and alpha2 contains a catalytic site. The alpha2/2 homodimer hydrolyzes PAF (1-O-alkyl-2-acetyl-sn-glycero-3-PC) and 1-O-alkyl-2-acetyl-sn-glycero-3-PE more efficiently, while the alpha1/1 homodimer and alpha1/2 heterodimer are more efficient toward 1-O-alkyl-2-acetyl-sn-glycero-3-PA than PAF. The alpha 1 subunit is reported to be up-regulated in primary human breast tumors and correlated with poor prognosis. Inactivation of alpha 1 subunit enhances cellular level of tumor-suppressing signaling lipids.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PAFAH 1B3 Antibody, clone 3E4.1 is an antibody against PAFAH 1B3 for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected PAFAH1B3 in A431, HeLa, HUVECs and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment from the C-terminal half of human PAFAH1B3.
      EpitopeInternal (C-terminal half).
      Clone3E4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3E4.1 detects the alpha 1 subunit found in the heterotrimeric type I intracellular PAF-AH. The alpha 1 subunit was originally named the gamma subunit (Arai, H., et al. (2002) J. Biochem. 131(5):635-640; Hattori, M., et al. (1993) J. Biol. Chem. 268(25):18748-18753).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PAFAH1B3
      • PAFAHG
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~30 kDa observed. 25.73 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAFAH1B3 in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1188 04055977332209