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MABS1326 Anti-Importin alpha-3/Qip1 Antibody, clone 3D10

Detect Importin subunit alpha-3/Qip1 using this rat monoclonal Anti-Importin alpha-3/Qip1, clone 3D10, Cat. No. MABS1326, validated for use in Western Blotting.

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Research Category: Signaling Gene Symbol: Kpna4, Qip1 UniProt Number: O35343
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MABS1326
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, B, Ht, R, MWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1326
      DescriptionAnti-Importin alpha-3/Qip1 Antibody, clone 3D10
      Alternate Names
      • Importin subunit alpha-3
      • Importin alpha Q1
      • Karyopherin subunit alpha-4
      • Qip1
      Background InformationImportin subunit alpha-3 (UniProt O35343; also known as Importin alpha Q1, Karyopherin subunit alpha-4, Qip1) is encoded by the Kpna4 (also known as Qip1) gene (Gene ID 16649) in murine species. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. Qip1/KPNA4 belongs to the importin- /karyopherin- (KPNA) family of adaptor proteins that interact with both monopartite and bipartite types of classical nuclear localization signals (cNLSs). The seven KPNA family members are divided into the 1 (KPNA1/5/6), 2 (KPNA2/7), and 3 (KPNA3/4) subfamilies based on structural and functional similarities. KPNA members mediate substrate cargo nuclear import by bridging substrate cargo and the Ran-karyopherin- complex. Both KPNA3 and Qip1/KPNA4 are reported to target methyl-CpG-binding protein 2 (MeCp2) bipartite NLS (255-RKAEADPQAIPKKRGRK-271). Qip1/KPNA4 can ineract with both wild-type and MeCp2 R270X mutants found in Rett syndrome (RTT) patients, while KPNA3 binds only wild-type, but not MeCp2 R270X mutants. Human and murine Qip1 contains an NLS (a.a. 43-52) in its importin- -binding (IBB) domain (a.a. 2-58), followed by ten ARM domains (a.a. 66-485), one majore and one minor NLS-binding site (a.a. 137-229 and a.a. 306-394).
      References
      Product Information
      FormatPurified
      PresentationPurified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Importin subunit alpha-3/Qip1 using this rat monoclonal Anti-Importin alpha-3/Qip1, clone 3D10, Cat. No. MABS1326, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

      Western Blotting Analysis: A representative lot detected endogenous importin 3/Qip1 in human HeLa, monkey COS-7, mouse L-929, rat NRK , hamster BHK, and bovine MDBK cell lysates (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).
      Biological Information
      ImmunogenRecombinant full-length mouse Importin alpha-3/Qip1.
      Clone3D10
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 3D10 detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Monkey
      • Bovine
      • Hamster
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat, Monkey, Bovine, Hamster. Predicted to react with Canine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kpna4
      • Qip1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~58 kDa observed. 57.76 kDa (human) and 57.79 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1326 04054839057281