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324717 Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Endo-β-N-acetylglucosaminidase H, Endo H

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324717
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      Description
      OverviewRecombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324717
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase H, Endo H
      References
      ReferencesRao, V., et al. 1995. Structure 3, 449.
      Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
      Trumbly, R.J., et al. 1985. J. Biol. Chem. 260, 5683.
      Product Information
      CAS number52769-51-4
      Activity≥3 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥30 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      324717-200MIU 04055977215984