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345811 Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

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345811
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345811-50UL
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      Description
      OverviewRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
      Catalogue Number345811
      Brand Family Calbiochem®
      References
      ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
      Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
      Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
      Scudder, P., et al. 1983. Biochem. J. 213, 485.
      Product Information
      Activity≥14 U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
      EC number3.2.1.103
      FormLiquid
      FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥140 U/mg protein
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
      Global Trade Item Number
      Catalogue Number GTIN
      345811-50UL 04055977214970